Intended use
Fecal Occult Blood (FOB) Test (Colloidal Gold) is used for qualitative detection of hemoglobin in human feces at home for self-testing, or at all levels of medical institutions for testing, so as to provide auxiliary diagnosis for digestive tract haemorrhagic disease.
Test principle
This assay is a one-step lateral flow chromatographic immunoassay.The test strip consists of: 1) a burgundy colored conjugate pad containing mouse anti-hHb antibodies conjugated with colloidal gold and 2) anitrocellulose membrane strip containing a Test line (T-line) and a Control line (C-line). The T-line is coated with anti-hHb antibodies, and the C-line is coated with goat anti-mouse IgG antibodies.When an adequate volume of test specimen is dispensed into the sample well of the device, the test specimen migrates by capillary action across the test strip. If the concentration of hHb in the specimen is at or above 50 ng/ ml, the T-line appears as a visible burgundy line. If the concentration of hHb in the specimen is below the detectable level, no T-line develops.
Materials and Components
1.100 test devices (strips), each sealed in a foil pouch.
2.Fecal sample tube including 1.2ml dilute buffer (0.85% NaCL) for each test.
3.One package insert (instructions for use).
4.Timer.
5.Fecal container.
Product Information
Specification
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1pcs/box,5pcs/box,25pcs/box
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Specimen
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Stool
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Storage
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4-30℃
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Test Procedure
Bring all materials and specimens to room temperature (4-30°C)
1.Specimen Collection
Randomly pierce the fecal specimen with the swab or stick at least 5 different sites to pick up and put into the fecal collection tubes with buffer.
2.Testing
( 1 ) Stir the fecal collection container vigorously to ensure a good liquid suspension.
( 2 ) Remove the test strip from the sealed foil pouch and immerse the strip vertically into the processed samples with the arrow end pointing towards the sample at least 15 seconds. Do not immerse past the“MAX”Line.
( 3 ) Take the strip out and lay the strip flat on a clean, dry, non-absorbent surface.
3.Test Results
Wait for red colored bands to appear. The test should be read in approximately 10 minutes. Do not interpret results after 10 minutes.